Microbial and enzyme technology: An efficient and convenient method for MiniPrep analysis of recombinant plasmids
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چکیده
منابع مشابه
Microbial and enzyme technology: An efficient and convenient method for MiniPrep analysis of recombinant plasmids
Minipreparation (MiniPrep) analysis is an essential step for obtaining a recombinant plasmid that carries a DNA insert containing a gene of interest. The most commonly used method for this involves cultivation of transformed Escherichia coli (E. coli) in liquid medium, brief centrifugation for precipitation of bacterial pellets, and subsequent lysis of the pellets. This process is time-consumin...
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ژورنال
عنوان ژورنال: Journal of Biomedical Science and Engineering
سال: 2014
ISSN: 1937-6871,1937-688X
DOI: 10.4236/jbise.2014.73013